Novel mildewcidal composition and method of use



United States Patent 3,506,766 NOVEL MILDEWCIDAL COMPOSITION AND METHOD OF USE Donald R. Arnold, Lincolndale, N.Y., and Anthony A.

Sousa, Raleigh, N.C., assignors to Union Carbide Corporation, a corporation of New York No Drawing. Application Mar. 23, 1966, Ser. No. 536,645, now Patent No. 3,418,330, dated Dec. 24, 1968, which is a continuation-in-part of application Ser. N 0. 324,183, Nov. 18, 1963. Divided and this application Aug. 7, 1968, Ser. No. 750,744

Int. Cl. A01n 9/22 US. Cl. 424263 6 Claims ABSTRACT OF THE DISCLOSURE Novel compositions containing an oxetane, such as 2- (meta-pyridyl)-2-phenyl-3,3-dimethyloxetane, as the active ingredient, are useful for controlling mildew.

This application is a divisional of US. patent application Ser. No. 536,645, entitled Novel Pyridyl Substituted Oxetanes and Derivatives Thereof filed Mar. 23, 1966, by D. R. Arnold and A. A. Sousa now US. Patent 3,418,330. Ser. No. 536,645 is a continuation-in-part of US. patent application Ser. No. 324,183, entitled Novel Oxetanes and Use Thereof filed Nov. 18, 1963, by D. R. Arnold and A. A. Sousa, now abandoned.

The present invention is directed to novel heterocyclic organic compounds and to a process for their preparation. In one aspect, this invention is directed to novel oxetanes. In a further aspect, this invention relates to certain novel oxetanes which are useful in fungicidal applications, particularly, as mildewcides.

Heretofore, a wide variety of synthetic methods have been reported in the literature for the preparation of oxetanes. However, most of these methods are not very general and frequently require starting materials which are exceedingly difficult to prepare. Moreover, the reported synthetic methods rarely give high yields and hence are undesirable for large scale commercial production.

It has recently been discovered that a wide variety of novel oxetanes can be conveniently prepared by the photocycloaddition of carbonyl compounds to olefins. In many instances, the yields of the desired oxetanes are high, sometimes nearly quantitative. Moreover, the starting materials are readily available and hence oxetanes can readily be obtained which were difficult or impossible to prepare by classical methods.

Accordingly, one or more of the following objects will be achieved by the practice of the instant invention. It is an object of this invention to provide a class of novel oxetanes. Another object of this invention is to provide a class of novel oxetanes which are characterized by the presence of a heterocyclic group attached to one of the ring carbon atoms. A further object is to provide novel 2-pyridyl-2-phenyl-3,3-dihydrocarbyloxetanes. Another object is to provide certain novel oXetanes which are useful in agricultural and biological applications. A still further object of this invention is to provide certain novel oxetanes which are useful as mildewcides. Another object is to provide a process for the preparation of novel oxetanes. These ice The novel oxetanes of this invention can be represented by the structural formula:

1 wherein R represents hydrogen, halogen, a hydrocarbyl group, a hydroxyhydrocarbyl group, or two R s on the same carbon atom can together form an alkylene group, i.e.,

(2) R and R can together with the carbon atom to which they are attached represent the group:

wherein R, has the same value as above; B represents a sulfonyl group, i.e.,

O N S (l a lower alkylene group of from 1 to 2 carbon atoms, or an alkyl-substituted lower alkylene group of from 2 to 10 carbon atoms, (3) R and R can together with the carbon atom to which they are attached represent the group:

and (4) at least one of R R represents a group other than hydrogen, hydrocarbyl, or haloaryl.

Preferred novel oxetanes encompassed by the aforementioned formula are those wherein R R R and R individually represent hydrogen, alkyl, alkenyl, aryl, alkaryl, aralkyl, cycloalkyl, cycloalkenyl, bicycloalkyl, bicyclo-alkenyl, haloalkyl, haloaryl, halocycloalkyl, alkoxyalkyl, alkoxyaryl, aminoalkyl, aminoaryl, alkylaminoalkyl, dialkylaminoalkyl, dialkylaminoalkyl, and

' the like, provide that no more than two of said R R., variables are hydrogen; R and R individually represent alkyl, alkenyl, aryl, alkaryl, aralkyl, cycloalkyl, bicycloalkyl, haloalkyl, haloaryl, haloalkaryl, halocarbonyl, alkoxyaryl, aminoalkyl, aminoaryl, alkylaminoalkyl, dialkylaminoalkyl, dialkylaminoalkoxyalkyl, pyridyl (ortho, meta and para), alkylpyridyl, piperidyl, piperidylalkyl, alkylpiperidyl, piperidinoalkyl, pyrimidyl, alkylpyrimidyl, pyrimidinylalkyl, pyrazolyl, pyrazylalkyl, N alkoxycarbonylpiperazinylalkyl, piperizinyl, N alkylpiperazinylalkyl, morpholinyl, alkylmorpholinyl, 1,2,5,6- tetrahydropyridyl, N alkyl 1,2,5,6 tetrahydropyridyl, N aryl 1,2,5,6 tetrahydropyridyl, N aralkyl 1,2,5,6- tetrahydropyridyl, carbamyl, alkylcarbamyl, cycloalkylcarbamyl, N-heterocycliccarbamyl and the like.

Particularly preferred oxetanes of this invention are those wherein each of the R1Rq variables contains up to 18 carbon atoms, and wherein at least one of the R and R variables is a heterocyclic group composed of carbon, hydrogen, and from 1 to 2 ring nitrogen and/or oxygen atoms.

Illustrative novel oxetanes encompassed by the aforementioned formula include, among others, the 2- heterocycl-2,3,3-trihydrocarbyloxetanes, e.g., 2-(ortho-pyridyl)-2-phenyl-3,3-dimethyloxetane, 2-(metapyridyl)-2-phenyl3,3-dirnethyloxetane,

2- (para-pyridyl) -2-phenyl-3 ,3-dimethyloxetane,

2- (meta-pyridyl -2, 3 ,3 -trimethyloxetane,

2- (ortho-pyridyl) -2, 3 ,3 -trimethyloxetane, 2-(para-pyridyl)-2,3,3-trimethyloxetane,

2- (para-pyridyl) -2phenyl-3 ,3 ,4,4-tetramethyloxetane,

and the like.

The novel oxetanes of this invention can be produced by the photocatalyzed reaction of a ketone with an olefinic compound. The reaction producing the novel oxetanes of this invention is carried out by bringing the ketone and the olefinic compound into admixture in a suitable reactor and irradiating the mixture with light energy of catalytic wavelength, typically in the range of from about 2,000 to about 4,000 angstroms. The use of light energy of shorter wavelengths substantially below 2,000 angstroms may engender the photolytic decomposition of the reactants and/or the oxetane product and is therefore to be avoided, while little if any of the desired reaction occurs using light energy of longer wavelengths substantially above 4,000 angstroms. Thus, the term light energy, as employed herein, contemplates wavelengths predominantly in the ultra violet spectrum. Convenient sources of such light energy include, for instance, tungsten bulbs, daylight, mercury vapor and xenon are lamps, etc.

The reaction of the ketone with the olefinic compound can be carried out in solution using an inert, normally liquid solvent such as a saturated aliphatic or aromatic hydrocarbon or halogen derivative thereof, as for instance, heptane, hexane, pentane, benzene, acetic acid, acetonitrile, carbon tetrachloride, and the like, especially those having a boiling point below about 100 C. The use of such a solvent is preferred. The reaction can, however, also be carried out neat, i.e., in the absence of external solvent.

The proportion in which the reactants are utilized can vary broadly, and does not limit the invention. Typically, the reactants are employed in a proportion of from about 0.1 mole to about 10 moles of the ketone per mole of the olefinic compound. Higher or lower proportions of reactants can also be employed satisfactorily. However, the efiicient utilization of the reactants will generally decrease when greater than stoichiometric, i.e., equimolar, proportions are employed.

The reaction temperature can also vary broadly typically in the range of from about 0 C. to about 100 C., and preferably in the range of from about 10 C. to about 30 C. Here again, higher or lower reaction temperatures may also be employed satisfactorily. In any given instance, however, the temperature should not be so high as to engender the decomposition of the relatively heat-sensitive oxetane product, and is dependent to a large extent upon the identity of any external solvent employed. Thus, the temperature should not be so high as to volatilize the solvent, nor so low as to preclude its normally liquid form. Preferably, the temperature should also be consistent with the dissolution of any gaseous reactant in the solvent, i.e., should not volatilize the reactant from the solvent.

When within the above temperature range, the reaction is generally carried out, i.e., irradiation continued, for a period of from several hours to several days depending upon the concentration of reactants present, the wavelength and intensity of the light energy employed, etc. Longer or shorter reaction periods sufiicient to produce the desired oxetane can also be utilized. Preferably, a stoichiometric amount or excess of the olefinic compound is admixed with the ketone and the reaction is carried out to completion as determined by periodically removing aliquots from the reaction mixture and subjecting the aliquots to infra-red analysis. Under such circumstances the completion of the reaction is indicated by the disappearance of the carbonyl peak in the infra-red spectrum, carbonyl absorption occurring at a wavelength of approximately 6 microns.

Any suitable vessel which will permit the transmission of light energy of the desired wavelength, as described above, can be employed as a reactor. Typically, Pyrex or quartz vessels are employed in this regard, Pyrex being preferred interposed between the reaction mixture and the source of light energy.

Upon completion of the reaction, the oxetane product can be recovered in any convenient manner. For instance, the product can be recovered as the residue obtained upon the evaporation or distillation of any unreacted material and/or solvent present. The product can thereafter be purified, if desired, by extraction or recrystalliza,- tion, etc.

The apparatus employed in each of the examples consisted of a quartz well (Hanovia No. 19434) with a Pyrex filter immersed in a Pyrex reaction vessel having a capacity of 200 milliliters. The reaction vessel was equipped at the bottom with a glass fitted gas inlet for the introduction of purge gas and gaseous reaction, and the top with two outlets, one protected by a mercury bubbler and the other covered by a rubber septum through which periodic withdrawal of aliquots of the reaction mixture could be made. The light source was a 450 watt (Hanovia No. 679A-36) mercury arc lamp. The apparatus was maintained at a temperature in the range of 5l0 C. by immersing it in a refrigerated bath and circulating cooling water through the well.

Unless indicated otherwise, all of the oxetane products described herein were prepared by essentially the same procedure using the appropriate ketone and olefinic compound as reactants. The elemental analysis results are also indicated in Table A for each example. The oxetane structure of the products was confirmed in each instance by infra-red analysis, oxetane absorption occurring at a wavelength of 10:0.3 microns, and nuclear magnetic resonance.

EXPERIMENTAL PROCEDURE A solution of 18.2 grams (0.1 mole) of benzophenone in 150 milliliters of benzene in the apparatus described above was purged with nitrogen after which isobutylene was introduced until the volume of the reaction mixture was 200 milliliters. The light source was turned on for a period of 24 hours. The excess isobutylene and the solvent was evaporated under reduced pressure leaving a colorless crystalline material (22.1 grams, melting point -85 C.) showing strong oxetane and no carbonyl absorption in the infra-red spectrum. The nuclear magnetic resonance spectrum of this material was consistent with 3,3-dimethyl-1,2-diphenyloxetane. Further purification was affected by chromatography on alumina eluting with n-hexane. The oxetane came oil in the first fractions. Recrystallization from ethanol afforded pure 3,3-dimethyl- 2,2-diphenyloxetane having a melting point of 88.5-90" C. In a similar manner, the carbonyl compounds and olewas removed on a rotary evaporation leaving a yellow oil which showed strong infra-red absorption at 10 due to the oxetane and no detectable carbonyl absorption. The yellow oil was chromatographed on an alumina column eluting with n-hexane-benzene mixture to give the pure fins set forth in Table A were reacted to give the desired 5 products listed as Examples 13, 14, and 15. oxetanes. In those instances wherein the reaction of the carbonyl The photocycloaddition of carbonyl compounds with compounds and olefins resulted in a mixture of isomeric the allenes was effected as follows: products, separation was effected according to known A solution of 18.22 grams (0.10 mole) of benzophenone 10 techniques to provide the pure isomers. and 11.54 grams (0.12 mole) of tetramethylallene in 170 It should also be noted that a wide variety of derivamilliliters of benzene was irradiated with a 450 watt mertives can be prepared from various oxetanes of this incury lamp in the same apparatus previously employed. vcntion. For example, the oxetane of Example 16 can The mixture was purged with nitrogen throughout the serve as the starting material for the preparation of acids, irradiation period. After twenty-four hours the solvent amines, and similar products.

TABLEA Elemental analysis in Percent Yield Calculated Found 111 Ex. Carbonyl compound Olefin Oxetane percent 0 H N C H N 1 Ortho-benzoyl- Isobutylene O'--Hz 57 80.30 7.16 5.85 80.11 7.09 5.83

pyridine. I

CaHs I s)2 2-C5H4N 2 Meta'benzoyldo OHz 48 80.30 7.16 5.85 80.18 7.16 5.96

pyridine.

C5115 Hs)2 3-C5H4N 3 Para-benzoylpyridine do OH 56 80.30 7.16 5.85 80.19 7.30 5. 94

CoHa --(CH2)1 4C5H4N 4 Meta-acetylpyridlne do 0 E2 "i5 3-'CBH4N (CH1):

5 Para-acetylpyridine do O [Eh 56 74.54 8.53 74.25 8.65

4C5H4N a)2 6 Meta-benzoylpyridine. Tetramethylethylene O--"|(CHa)2 28 80.86 7.92 80.50 8.07

CaHs s)2 3-CaH4N 7 .Anthrone Isobutylene 1'1 55 86.36 7.25 86.01 7.38

8 Dibenzsuberone .do H 96 86.32 7.63 86.26 7.76

Elemental analysis in Percent Yield Calculated Found 1n Ex. Carbonyl compound Olefin Oxetane percent 0 H N C H N 9 1,3-dibenzoylbenzene ..do H2 GHE O 10,, B enzophenone l Dimethylallene 0 C Hz) 2 16 CnHs) 2-%O H2- (Ca s) 2 11 ..do ..do (CaH6)2I I( a)2 39 12 d0 C(CHS)2 2 CH2 (CaH5)2 3)2 13 .do Tetramethyl allene 0 (CH3) 2 17 86. 28 7. 97 86. 49 8. 14

14 "do --dO (CeH5)2-\ |(CHs)2 22 86. 05 7. 00 86. 18 7. 08

O (C 0H5) 2 15 "do ..do (CoH5)zl'-(CHa)2 43 86-05 7.00 85.79 6. 94

CH3) 2 C 6H5) 2-() 16 Phenylglyoxylamide- Isobutylene OH2 C 6H5 CH3) 2 O NH:

17 Ortho-benzoyll-carbethoxy- C------- (011:) z

pyridine. 2,2-dimethyl- I 3-isobutenyl- 0 0 st cyclopropane. C 6H4Nf- (CH3) 2 18.... Benzophenone Isoprene sulfone 56 68. 76 5. 77 68. 58 5. 92

S 02 (CsHs) z- HsC 19. Trifluomethyl- Isobutylene C (C1102 79 acetopheuone.

lus

CaHa

Elemental analysis in Percent Yield Calculated Found 1n Ex. Carbonyl compound Olefin Oxetane percent H N C H N Para-cyanoaeetodo C--Hg 35 phenone.

(i-ON) CaHq (CHa)2 21 Ortho-trifiuoro- ..-do 0 H2 72 70.57 5.60 70.70 5.55

methylbenzophenone.

CoHs '-'(CH;)2

(2-C Fa) CeH4 22 Para-trifluorodo 2 87 70.57 5.60 70.90 5.66

methylbenzophenone.

all

( zlz F3) CGHA The novel oxetanes of this invention may be employed in a variety of fungicidal applications, salient among which is their use as mildewcides. Found to be particularly useful in this regard are the meta-pyridyl substituted oxetanes. The meta-pyridyl substituted oxetanes generally demonstrate a high degree of mildewcidal effectiveness at lower concentrations, or when employed at equal strength demonstrate superior mildewcidal effectiveness, as compared with the other oxetanes contemplated by this invention.

It has also been found that the novel oxetanes of this invention are especially effective against Erysipthwz polygon i, the causal organism of powdery mildew of bean and Podosphaerai leucotricha, the casual organism of powdery mildew of apples. Significantly, when applied to a mildew succeptible host in a suitable inert carrier, the oxetanes, and particularly the meta-pyridyl substituted oxetanes, demonstrate mildewcidal activity at concentrations as low as 0.1 part per million parts of carrier, or even at somewhat lower concentrations. Moreover, the oxetanes demonstrate good retention of mildewcidal activity for appreciable periods of time following their formation.

Typically, the oxetanes are employed as mildewcides in a concentration in the range of from about 4 to about 500 parts per million parts of carrier, although higher or lower mildewcidally effective amounts can also be employed. A preferred range is from about 20 to about 100 parts per million parts of carrier. Any suitable inert liquid or solid carrier such as water, talc, or the like, can be employed in this regard, as can readily be determined by one skilled in the art in light of this disclosure.

The procedure employed in evaluating the novel oxetanes of this invention is as follows:

BIOLOGICAL TEST PROCEDURES A stock suspension of the oxetane was prepared by dissolving one gram in 50 milliliters of acetone in which had been dissolved 0.1 gram of octylphenoxy polyethoxyethanol as an emulsifying agent. The resulting solution was mixed into 150 milliliters of water to give 200 milliliters of a suspension containing the oxetane in finely divided form. The various test concentrations described in parts per million were prepared by dilution of this stock suspension.

Tender green beans Were used as the host plant for the bean mildew, Erysiphe polygoni. A culture of this organism Was maintained on bean plants in a greenhouse. Twenty-four hours prior to testing, uninfected plants with primary leaves fully expanded were inoculated by brushing their leaves lightly with plants taken from the stock culture.

The bean plants infected with mildew were sprayed on a revolving turntable for 30 seconds at a pressure of 40 p.s.i.g. Approximately 100 milliliters of spray were delivered. For control purpose, an equal volume of a water solution containing acetone emulsifier, and a con- Ventional mildewcide, Karathane in the same concentrations as the stock suspension above was also sprayed on other infected plants.

After the spray had dried, the plants were held in a greenhouse for a period of 7-l0 days. At the end of this period, visual observations of mildew control were made according to the following designations:

5=100% control, no spots per leaf 4:1-3 spots per leaf 3=4-10 spots per leaf 2:Many but distinctly different spots 1=Leaf overrun with mildew; equal to check plants.

In a similar procedure, Maclntosh apple seedlings were used as the host plant for the apple mildew, Podosphaera leucotricha. The seedlings were inoculated twenty-four hours prior to initial spraying with a stock solution prepared as described above. Spraying was carried out as described above at weekly intervals for a period of three weeks. Visual observations of mildew control were made one week following each application in accordance with the designations set forth above. The treated plants were placed among untreated, heavily mildewed apple seedlings during the entire test period except for the time required for spraying,

The effective dose required for a 3 rating in the control of bean mildew was obtained for the oxetanes by plotting log concentration of oxetane versus probit rating and deriving the concentration of oxetane in parts per million parts of water required for 50 percent control of mildew. Thus derived, the ED;, value, for example, for 2-(meta-pyridyl)-2-phenyl 3,3 dimethyloxetane, was found to be 15.

In addition to contacting the causative organism, mildew control is also realized by the application of the oxetanes of this invention in similar manner to an uninfected mildew succeptible host. Protection against the onset of mildew is thereby realized for appreciable periods of time during which the oxetanes are active as mildewcides.

Moreover, it has been found from screening tests that the oxetanes of this invention, particularly the metapyridyl substituted oxetanes, are effective in other fungicidal applications. By way of illustration, 2-(meta-pyri- 1 2,4-dinitro-6-(2-0ctyl)phenyl crotonate.

disclosed. Various modifications and embodiments of this invention can be made without departing from the spirit and scope thereof.

What is claimed is:

1. A mildewcidal composition comprising, in an inert carrier, as the active mildewcidal ingredient, an oxetane of the formula:

an, I

wherein R is pyridyl.

2. The mildewcidal composition according to claim 1 wherein said oxetane is 2-(meta-pyridyl)-2-phenyl-3,3- dimethyloxetane.

3. A method for controlling mildew which comprises contacting the mildew causative organism with a mildewcidal composition comprising an inert carrier and, as the active mildewcidal ingredient, an oxetane of the formula:

formula:

0 H CJ R wherein R is pyridyl.

6. The method according to claim 5 wherein said oxetane is Z-(meta pyridyl) 2 phenyl-3,3-dimethyloxetane.

References Cited UNITED STATES PATENTS 3,146,180 8/1964 Cenci 204162 ALBERT T. MEYERS, Primary Examiner A. J. ROBINSON, Assistant Examiner 

